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She gets attacked by a man after getting tricked and as hes about to presumably kill her as shes on the ground she punches him in the balls. Show is on Amazon Prime. Happens at about 23:35 into episode 1 Season 1
At 38:50 she knees a guy in front of the night club in panic. ( onscreen ) Not a memorable bust but the actress is hot and the reaction is good.
Ilana Glazer kicks the villain in the balls near the end.
After being seduced by a fellow student, the familiar gets kneed by her master then has his head stomped on Episode 2, halfway through
Season 10: Episode 10.At 19:42. After Quagmire tries to sleep with her daughter,Lois says,"if you try to touch my daughter again I will cut your thing off and feed it to Brian!" (Their dog)
http://wfgo.net/movie/180-Family_Guy_1010_Quagmire_and_Meg.html
S14 Ep11
Lois says "that woman just smiled when Stewie banged his balls on the floor".
In season 3 episode 3...about 19:00 into the episode Lois kicks Peter in the nuts briefly, but still a bust. Also earlier in the episode
NO KICK IN THAT EPISODE
However there is one in episode 307 at 20.10 by Lois
Episode 3 she kicks a guy with gun around 3 mins and no reaction
season 2 episode 8 vendetta at time 13 minutes and 8 seconds in offscreen kick